pPAmCherry-Mito Vector

Description

pPAmCherry-Mito Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to the mitochondrial targeting sequence derived from the precursor subunit VIII of human cytochrome C oxidase. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells.HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates corresponding to 30,000 cells were prepared from HEK 293 cells transiently expressing PAmCherry-N1 Lane 1 or a negative control untransfected cells; Lane 2 . Both lysates and a positive control 5 ng recombinant mCherry; Lane 3 were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back Back Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria.U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated using an Argon 458 nm laser scan speed: 400 Hz , and the cells were imaged using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set. Back Back PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria.Activating the mitochondria in just one region of the cell makes it possible to follow their movements into dark nonactivated areas of the cell. U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated in a small region of a cell, and the cells were imaged every 10 sec for 15 min using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set. Back