PPOX-Arg38 Stable Knockout U-2 OS Cell Line (PPOXArg38: R38/1)

Description

The knockout of Arg38 in the PPOX gene in this U-2 OS cell line, using CRISPR/Cas9 genome editing technology, leads to a complete loss of PPOX enzymatic activity, showcasing the cellular effects of inhibited heme biosynthesis. Characterized by a stark increase in protoporphyrinogen IX levels and reduced heme content, these cells maintain normal proliferation rates but exhibit compromised mitochondrial respiratory function. This cell line serves as a vital resource for probing into the cellular mechanisms underpinning heme biosynthesis disorders and evaluating potential pharmacological interventions. Complete list of cell lines from this panel: Cat. T3477 - PPOX-Cys459 Stable Knockout U-2 OS Cell Line PPOXCys459: C459/1 Cat. T3478 - PPOX-Arg38 Stable Knockout U-2 OS Cell Line PPOXArg38: R38/1 Cat. T3479 - PPOX-Trp227 Stable Knockout U-2 OS Cell Line PPOXTrp227: W227/1