pRetroQ-mCherry-N1 Vector

Description

The pRetroQ-mCherry-N1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry;a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed mRFP1 by site-directed mutagenesis. Inserting a cDNA in the MCS upstream of the mCherry coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System Cat. No. 631530 . Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Retro-X retroviral vector maps.IRES-containing bicistronic retroviral vectors allow you to express two target genes Vector A: pQCXIX or a target gene and an antibiotic resistance gene Vector B: pQCXIH,pQCXIN and pQCXIP . Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1 . Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, Vectors DandE: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ . To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP .