pRetroX-G1-Red Vector

Description

pRetroX-G1-Red is a self-inactivating retroviral vector that expresses mCherry-hCdt1 30120 , a fluorescent, ubiquitination-based, cell-cycle indicator Fucci . The vector allows you to identify cells that are in G1 phase. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Cell-cycle progression as visualized with Fucci probes.Successive rounds of division in HEK 293 Tet-On Advanced cells were monitored using Fucci probes expressed from the pTRE-CellCycle vector in the presence of doxycycline Dox . As the cells progressed through the cell cycle, their nuclei varied in color: cells lacking color were transitioning between phases M and G1. The nuclei of cells in G1 phase were then labeled with the red fluorescent protein mCherry. As the cells transitioned between phases G1 and S, they appeared light pink/violet. Finally, the nuclei of cells in S through M phases were labeled with the cyan fluorescent protein AmCyan1. Back Retroviral delivery is available for a variety of cell-cycle Fucci probes.Fucci probes containing Geminin-fluorescent protein fp fusions are visible in phases S through M, whereas the probe containing Cdt1-fp expressed by pRetroX-G1-Red is visible during the G1 phase of the cell cycle. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M. Back Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle.pTRE-CellCycle is a bidirectional, tetracycline Tet -inducible expression vector that lets you inducibly express Fucci probes during all phases of the cell cycle Panel A . In cells transfected with the vector, the Cdt1 fusion is visible through G1 phase, while the Geminin fusion is visible from S through M phases Panel B . A fluorescence micrograph of HEK 293 Tet-On Advanced cells transfected with the pTRE-CellCycle vector and cultured in the presence of Dox is shown inPanel C. Back Movie of cell-cycle progression, visualized with Fucci probes.No color: cells transitioning between phases M and G1. Red: cells in G1 phase. Light pink/violet: cells transitioning between phases G1 and S. Cyan: the nuclei of cells in phases S through M. Back Back