pRetroX-IRES-DsredExpress Vector

Description

The pRetroX-IRES-DsRedExpress vector is a bicistronic retroviral expression vector containing DsRed-Express as a marker for transfection/infection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus EMCV internal ribosomal entry site IRES . The IRES sequence allows the protein of interest and DsRed-Express to be independently translated from the same mRNA transcript. DsRed-Express is a human codon-optimized variant of theDiscosomasp. red fluorescent protein DsRed that has been engineered for faster maturation. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Retro-X retroviral vector maps.IRES-containing bicistronic retroviral vectors allow you to express two target genes Vector A: pQCXIX or a target gene and an antibiotic resistance gene Vector B: pQCXIH,pQCXIN and pQCXIP . Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1 . Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, Vectors DandE: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ . To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP . Back Mechanism of IRES bicistronic expression from Retro-X retroviral vectors.The internal ribosome entry site IRES permits a protein of interest and a fluorescent protein to be independently translated from the same mRNA. SD = Splice Donor. SA = Splice Acceptor. = packaging signal. LTR = long terminal repeat. Back