pRetroX-Tight-Hyg Vector

Description

pRetroX-Tight-Hyg is a retroviral vector that expresses a gene of interest from the Tet-responsive element TRE upon activation by either the tTA or rtTA regulatory protein. It can be used with any established Tet-On or Tet-Off cell line, or in conjunction with the regulatory plasmids from any Tet or Retro-X Tet System. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Retroviral delivery combined with tetracycline-inducible expression.HeLa cells stably transduced with Retro-X Tet-Off Advanced retrovirus were subsequently infected with a Retro-X-Tight-Pur retrovirus engineered for inducible luciferase expression. Infected cells were grown in the absence or presence + of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units. Back Each Retro-X Tet-Advanced system consists of two retroviral vectors.A regulator vector e.g., pRetroX-Tet-On Advanced expresses an optimized tetracycline-controlled transactivator. The response vector pRetroX-Tight-Pur contains an improved tetracycline response element TRE within thePTightpromoter that controls expression of your gene of interest.