Primary Tumor Cell Line (T1-A )

Description

This highly tumorigenic cell line is derived from a primary tumor generated by the s.c. injection of GhrasT-NIH/3T3 Cat. T3317 cells into the hindquarters of an outbred NIH Swiss mouse. Note: Identical injections with this T1-A cell line produced 25x more primary tumors 20 of 20 = 100 % mice injected than were produced with injections of its parental transformed GhrasT-NIH/3T3 cell line 4 of 104 = 3.8 % mice injected . This cell line is part of an eight cell R/J Model of Metastatic Progression designed using the NIH Swiss mice species to acquire isogenic cell types T4138, T8981,T3317, T8144-T8148 that capture genetic/phenotypic stages in the progression to highly metastatic growth within this same species. Compared to some other models, the metastasis related changes captured in this unique progression model are easier to discern above their less noisy isogenic background and have progressed within their native NIH Swiss in vivo micro-environments. This isogenic R/J Metastasis Cell Model was produced by an in vitro/in vitro six-step progression from mortal normal NIH Swiss mouse cells Cat. T4138 that were used to produce immortal pre-cancer NIH/3T3 cells Cat. T8981 that were transfected with the human HRAS oncogene to produce transformed cancer GhrasT-NIH/3T3 cells Cat. T3317 . Then beginning with a series of in vivo/in vitro progressive transfers of GhrasT-NIH/3T3 cells produced primary tumor T1-A cells Cat. T8144 that produced local metastatic lesion T2-A cells Cat. T8145 that simultaneously produced twin distant metastatic lung lesion T3-PA cells Cat. T8146 with its twin distant metastatic liver lesion cells Cat. T1847 . The liver derived cells produced highly distant metastatic lung lesion cells Cat T1848 which retained the human HRAS oncogene and they demonstrated a highly metastatic phenotype as they produced wide-spread simultaneous external and internal metastases in multiple sites in multiple mice when injected i.v. into nude NIH Swiss mice. Complete list of cells included in the R/J Model: Cat. T4138 - Normal Primary Diploid Mortal NIH Swiss Mouse Embryonic Cells Cat. T8981 - NIH-3T3 Cells Cat. T3317 - HRAS Stably Expressing NIH/3T3 Cell Line GhrasT-NIH/3T3 Cat. T8144 - Primary Tumor Cell Line T1-A Cat. T8145 - Local Metastasis Tumor Cell Line T2-A Cat. T8146 - Distant Pulmonary Metastasis Tumor Cell Line T3-PA Cat. T8147 - Distant Hepatic Metastasis Tumor Cell Line T3-HA Cat. T8148 - Highly Metastatic Distant Pulmonary Tumor Cell Line T4-PA