PrimeSTAR Max DNA Polymerase Ver.2

Description

PrimeSTAR Max DNA Polymerase Ver.2 is a high-performance PCR amplification system that offers the highest levels of fidelity and extension speed. With an improved success rate of target amplification in high-speed reactions compared to PrimeSTAR Max DNA Polymerase Cat. # R045A/B , PrimeSTAR Max DNA Polymerase Ver.2 maintains the fidelity, making it a fully compatible and superior alternative. As a 2X premixed reagent with an antibody-mediated hot start formulation to suppress DNA Polymerase activity and 35 exonuclease activity, it allows quick preparation of reactions at room temperature and is useful for high-throughput applications. Reaction size: 1 rxn equals a 50 mul total reaction volume. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back cDNA amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 compared with PrimeSTAR Max DNA Polymerase and high-fidelity enzymes from Company N and Company T.PrimeSTAR Max DNA Polymerase Ver.2 Cat. # R047A , PrimeSTAR Max DNA Polymerase Cat. # R045A Panel A , and high-fidelity enzymes from Company N and Company T Panel B were used to amplify theTFRgene 4 kb from cDNA templates obtained from from 10 ng to 1,000 ng of total RNA 50 mul reaction volume under conditions recommended by the respective manufacturers. PrimeSTAR Max DNA Polymerase Ver.2 exhibited the strongest amplification of cDNA templates from 200 ng or greater of total RNA at a 5 sec/kb extention time PanelA, top , while increasing the extension time to 10 sec/kb Panel A, bottom resulted in improved amplification for cDNA templates obtained from 400 ng to 1 mug of total RNA. Back Genomic DNA amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 compared with PrimeSTAR Max DNA Polymerase and high-fidelity enzymes from Company N and Company T.PrimeSTAR Max DNA Polymerase Ver.2 Cat. # R047A , PrimeSTAR Max DNA Polymerase Cat. # R045A Panel A , and high-fidelity enzymes from Company N and Company T Panel B were used to amplify theDCLRE1Agene 4 kb using human genomic DNA gDNA; 0.1 ng to 1 mug in 50 mul reaction volume under conditions recommended by the respective manufacturers. PrimeSTAR Max DNA Polymerase Ver.2 exhibited the strongest amplification of gDNA templates Panel A, top and increasing the extension time from 5 sec/kb to 10 sec/kb Panel A, bottom resulted in improved amplification, specially for 600 ng to 1 mug of gDNA template. Back Long template amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 compared with PrimeSTAR Max DNA Polymerase and high-fidelity enzymes from Company N and Company T.Templates of lengths ranging from 0.5 kb to 15.0 kb were amplified from 100 ng human genomic DNA under conditions recommended by the respective manufacturers in 50 mul reaction volumes. Amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 Cat. # R047A was compared with PrimeSTAR Max DNA Polymerase Cat. # R045A PanelA and high-fidelity enzymes from Company N and Company T PanelB . PrimeSTAR Max DNA Polymerase Ver.2 exhibited the strongest amplification with an extension time of 5 sec/kb for templated under 6 kb and 10 sec/kb for templates over 6 kb. High-fidelity PCR enzymes from Company N and Company T required longer extension times, adding to the total reaction time to achieve comparable amplification PanelB . The total reaction time for amplifying a 15 kb template is indicated in green for PrimeSTART Max DNA Polymerase Ver.2 and PrimeSTAR Max DNA Polymerase and red for high-fidelity enzymes from Company N and Company T.