Pro Ligation-Free Cloning MasterMix

Description

abms Pro Ligation-Free Cloning MasterMix is a ready-to-use solution designed for rapid, efficient and seamless DNA assembly regardless of fragment length or end compatibility. It enables one-step assembly of multiple DNA fragments with overlapping ends in a single isothermal reaction making it ideal for synthetic biology and molecular biology applications. To support accurate fragment amplification prior to assembly, the kit includes MegaFi Pro Fidelity 2X PCR MasterMix to enable robust, high-fidelity PCR. Key Features: One-step cloning: Enables rapid construction of new vectors in a single reaction Scar-free cloning: Constructs are free of unwanted or extra base pairs Customizable: Modify your constructs with ease regardless of restriction site availability, fragment length or end compatibility Robust assembly: Increased efficiency for complex, multiple fragment DNA assemblies Efficient, low cost alternative to Gibson Assembly Product Component Quantity Pro Ligation-Free Cloning 2X MasterMix 500 mul Positive Control Insert 5 mul Positive Control Vector 5 mul MegaFi Pro Fidelity 2X PCR MasterMix 1.25 ml Customer Testimonial I tried the molecular cloning of inserting two DNA fragments 2Kb & 0.5Kb into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit. -Jianfeng Lan, The Buck Institute