Protein A ELISA Kit

Description

This ELISA kit is designed to overcome the low analytical recovery of Protein A encountered in many commercial kits. Most low recovery samples are either incomplete antibody fragments or Fc-fusion proteins, where the Fc fragment of antibody is linked to another non-antibody molecule. The properties of such unnatural drug substance constructs means that conventional sample treatment procedures are ineffective in dissociation, denaturation, precipitation, and removal of the drug substances prior to using the Protein A detection assay. Any un-denatured drug substance remaining in the sample after the treatments step can re-associate with leached Protein A, resulting in under-recovery by ELISA. The F400Z Protein A ELISA kit has been qualified using several problematic samples and found to yield acceptable recovery on all. This kit uses the same antibodies standards and sample treatment protocol as in the F400 ELISA Kit except it also contains a Recovery Enhancer solution that is combined with the HRP conjugated anti-Protein A antibody F401Z before use. The Recovery Enhancer prevents the re-association of any drug substance that was not completely removed during the sample treatment step. This F400Z kit accurately quantitates the unnatural construct of Protein A such as the MabSelect SuRe as well as other conserved, natural and recombinant Protein A constructs. Please use Sample Diluent, Item No. I028 , with these kits; available separately. Note: Newer Protein A assays, known as Mix-N-Go methods are also available, including a kit calibrated to Cytivas former GE Healthcares MabSelect SuRe F610 . Download our Mix-N-Go kit selection guide to learn more about our Protein A Mix-N-Go ELISA kits.