PSEUDOMONAS AGAR FOR DETECTION OF PYOCYANIN (as per IP)

Description

Pseudomonas Agar is based on the formulation described by King et al and as recommended by Indian Pharmacopoeia for detecting pyocyanin, a water soluble pigment by Pseudomonas species from clinical specimens such as stools, wounds, and urine. Pseudomonas species are commonly isolated pathogen and is the significant causative agent of nosocomial, skin and burn infections. Pseudomonas strains are reported to produce phenazine pigments like Pyocyanin- blue green redox-active secondary metabolite pigment, pyorubin-rust brown pigment, -oxyphenzine- a breakdown product of Pyocyanin, pyoverdin-a water soluble yellow green pigments also known as fluorescein. Pyocyanin is readily recovered in large quantities in sputum from patients with cystic ffibrosis, an infection caused by Pseudomonas. This medium enhances the formation of Pyocyanin and/or pyorubin and reduces that of fluorescein.