ptdTomato-N1 Vector

Description

ptdTomato-N1 is a mammalian expression vector containing a tandem sequence of the dimeric red fluorescent protein, Tomato. An MCS at the 5 end of the coding sequence makes it possible to insert a gene of interest in order to express it as an N-terminal fusion with tdTomato. The unmodified vector can be used to express tdTomato in mammalian cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Schematic for fluorescent fusion protein vectors.Panel A.N-terminal fluorescent protein FP vector.Panel B.C-terminal fluorescent protein vector. Back tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model.False-color overlay images regions of interest encircled indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence.Panel A.Implanted tube with 100 x 106MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec.Panel B.Implanted tube with 100 x 106MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec. Back