Raf Dominant-Negative Vector Set

Description

Set of three vectors for studying Raf or MAPK signaling pathways: the pCMV-Raf-1 Vector expresses the wild-type Raf protein, the pCMV-RafCAAX expresses a constitutively active form of the Raf protein, and the pCMV-RafS621A Vector expresses a mutated form of Ras with a Ser-to-Ala mutation at residue 621. These vectors can be used to study Raf-related signal transduction pathways in mammalian cells. Each gene is expressed from the constitutive CMV promoter. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Inhibition of NFkappaB activity using pCMV-IkappaBalpha and pCMVkappaIalphaBM from the IkappaB Dominant-Negative Vector Set Cat. # 631923 .HEK 293 cells were transiently transfected with the indicated plasmids. After 16 hr, fresh medium was added to the cells along with TNF to induce the NFkappaB pathway. SEAP activity was assayed 6 hr later to determine NFkappaB activity. Back