Random Primer DNA Labeling Kit Ver. 2

Description

The Random Primer DNA Labeling Kit, Version 2 is designed to yield DNA probes with high activity for hybridization and can be used to label DNA with 32P -alpha-, 35S -alpha- or 3H -alpha-dCTP. This kit is based on a modified method of Feinberg and Vogelstein that utilizes random oligonucleotide primers and the cloned exonuclease-free Klenow fragment ofE. coliDNA polymerase I. The use of longer 9-mer primers and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This method overcomes many of the disadvantages of the conventional nick translation procedure while producing probes from small amounts of DNA 10 to 20 ng . It can also be used to label DNA fragments that are embedded in low-melting temperature agarose gel slices. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back