RAPPAPORT VASSILIADIS R10 MEDIUM

Description

Rappaport Vassiliadis R10 Medium is a selective enrichment medium that is used following pre-enrichment of the specimen in a suitable pre-enrichment medium. It has gained approval for use in analyzing milk and milk products, raw flesh foods, highly contaminated foods and animal feeds. Rappaport et al formulated an enrichment medium for Salmonella that was modified by Vassiliadis et al. The Vassiliadis modification, designated R10/43?C, had a reduced level of malachite green and recommended incubation at 43?C. Later work by Peterz showed that incubation at 41.5 ? 0.5?C for 24 hours improved recovery of Salmonella species. Rappaport Vassiliadis R10 medium selectively enriches for salmonellae because bacteria, including other intestinal bacteria, are typically resistant to or inhibited by malachite green, high osmotic pressure and/or low pH. S. Typhi and S. Choleraesuis are sensitive to malachite green and may be inhibited.