Ready-To-GlowTM Automation Kit

Description

Our Ready-To-Glow Automation Kit is a complete detection system for analysis of gene activity via plate readers with automated injection systems. It is designed for use with a transcription reporter system based onMetridialuciferase activity in the supernatant of transfected cells. In the presence of secreted luciferase in the sample, a nonluminescent substrate is converted to a luminescent compound. The intensity of bioluminescence is directly proportional to the amount of luciferase in the sample. The kit includes substrate buffer, reaction buffer, and a lyophilized secreted luciferase substrate. It has been optimized to work with our Ready-To-Glow Secreted Luciferase Vector Kit Cat. No. 631729 . Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back Back Back Linear range of detection of Ready-To-Glow secretedMetridialuciferase from media supernatants.HEK 293 cells and CHO cells were transiently transfected with the pMetLuc-Control vector. Media samples were collected from the transfected cells and 1:10 serial dilutions were performed in DMEM containing 10 % FBS, to the concentrations noted. Ready-To-GlowMetridialuciferase activity was measured according to the luciferase assay protocol for either 96-well Panel A or 384-well Panel B plates. Back Use of secretedMetridialuciferase in a high-throughput screening HTS application.The figure shows a screen shot from a CCD camera of a 1,536-well plate containing stable CHO cells transfected with a forskolin-responsiveMetridialuciferase gene pASM-Lu164 . 300 cells/well were plated in a 1,536-well microtiter plate. Cells were incubated with increasing concentrations of forskolin and the plate was incubated for 4 hr at 37 C.Metridiasubstrate was added in increasing concentrations and the plate was visualized using a CCD camera system integration time: 60 sec . Courtesy of Bayer Health Care, Germany . This pseudocolor image reflects luminescence intensity. Yellow regions are the brightest, while black regions are the least bright. Back