Recombinant Human CD20 (C-Flag)

Description

EnkiLife provides the Recombinant Human CD20 (C-Flag) protein,aslo know as B-lymphocyte antigen CD20,B-lymphocyte surface antigen B1,Leukocyte surface antigen Leu-16,Membrane-spanning 4-domains subfamily A member 1,Bp35,CD20,MS4A1, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human B-lymphocyte antigen CD20 is produced by our Mammalian expression system and the target gene encoding Met1-Pro297 is expressed with a Flag tag at the C-terminus.*The product is not recommended for cell based experiments.Accession:P11836. Expression Host:Human cells.Species:Human.Predicted Molecular Mass:34.3 KDa. Background:CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG.