Recombinant Human CPA2 (C-6His)

Description

EnkiLife provides the Recombinant Human CPA2 (C-6His) protein,aslo know as Carboxypeptidase A2,CPA2, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human Carboxypeptidase A2 is produced by our Mammalian expression system and the target gene encoding Leu17-Tyr417 is expressed with a 6His tag at the C-terminus.Accession:AAP36067.1. Expression Host:Human Cells.Species:Human.Predicted Molecular Mass:45.91 KDa. Background:Carboxypeptidase A2 (CPA) is a secreted pancreatic procarboxy-peptidase that cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition.