Recombinant Human DNA PolymeraseBeta (C-6His)

Description

EnkiLife provides the Recombinant Human DNA PolymeraseBeta (C-6His) protein,aslo know as DNA Polymerase Beta,POLB, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human DNA Polymerase Beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus.Accession:P06746. Expression Host:E.coli.Species:Human.Predicted Molecular Mass:39.23 KDa. Background:Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5 sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3 end of the arising single-nucleotide gap. It conducts gap-filling DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.