Recombinant Human HGF

Description

EnkiLife provides the Recombinant Human HGF protein,aslo know as Hepatocyte growth factor, HPTA, HGF, SF, Scatter factor, Hepatopoietin-A, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:≤10 EU/mg;Construction:Recombinant Human HGF is produced by our Mammalian cell expression system and the target gene encoding α chain：Gln 32 -Arg 494 & β chain：Val 495-Ser 728 is expressed.Accession:P14210. Expression Host:Human Cells.Species:Human.Predicted Molecular Mass:53.7 kDa(αchain),26 kDa(βchain). Background:HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases and contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.