Recombinant Human IL-5RA (C-6His)

Description

EnkiLife provides the Recombinant Human IL-5RA (C-6His) protein,aslo know as Interleukin-5 receptor subunit alpha,IL-5 receptor subunit alpha,IL-5R subunit alpha,IL-5R-alpha,IL-5RA,CDw125,CD125,IL5RA,IL5R, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human Interleukin-5 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Asp21-Glu335 is expressed with a 6His tag at the C-terminus.Accession:Q01344. Expression Host:Human Cells.Species:Human.Predicted Molecular Mass:36.7 KDa. Background:Interleukin-5 Receptor alpha (IL-5Rα, CD125) is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. Mature human IL-5 Rα consists of a 322 aa extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 20 aa transmembrane segment, and a 58 aa cytoplasmic domain. Within the ECD, human IL-5Rα shares 71% aa sequence identity with mouse and rat IL-5 Rα. Alternate splicing of human IL-5 Rα generates soluble secreted forms which function as IL-5 antagonists. The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL-5 Rα and the transmembrane common β chain (βc/CD131) which is shared with the receptor complexes for IL-3 and GMCSF. IL-5 Rα binds IL-5 at low affinity and then associates with preformed βc oligomers to form the signaling competent receptor complex. IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the up-regulation of transmembrane IL-5Rα followed by eosinophilic differentiation and activation.