Recombinant Human Legumain (C-6His)

Description

EnkiLife provides the Recombinant Human Legumain (C-6His) protein,aslo know as Legumain,Asparaginyl Endopeptidase,Protease Cysteine 1,LGMN,PRSC1, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Ile18-Tyr433 is expressed with a 6His tag at the C-terminus.The enzyme achieves its activity under acidic pH.Accession:AAH03061.1. Expression Host:Human Cells.Species:Human.Predicted Molecular Mass:48.72 KDa. Background:Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.