Recombinant Human MANF (C-6His)

Description

EnkiLife provides the Recombinant Human MANF (C-6His) protein,aslo know as Mesencephalic astrocyte-derived neurotrophic factor,Arginine-rich protein,Protein ARMET,ARMET,ARP, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human Mesencephalic Astrocyte-derived Neurotrophic Factor is produced by our Mammalian expression system and the target gene encoding Leu25-Leu182 is expressed with a 6His tag at the C-terminus.Accession:P55145. Expression Host:Human Cells.Species:Human.Predicted Molecular Mass:18.99 KDa. Background:Mesencephalic astrocyte-derived neurotrophic factor(MANF) is a secreted protein which belongs to the ARMET family. MANF selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. MANF enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. MANF inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF and a homologous protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. MANF expression was widespread in the nervous system and non-neuronal tissues.