Recombinant Human MGMT (N-6His)

Description

EnkiLife provides the Recombinant Human MGMT (N-6His) protein,aslo know as Methylated-DNA--protein-cysteine methyltransferase,6-O-methylguanine-DNAmethyltransferase;O-6-methylguanine-DNA-alkyltransferase,MGMT, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human Methylated-DNA-protein-cysteine Methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.Accession:P16455. Expression Host:E.coli.Species:Human.Predicted Molecular Mass:23.8 KDa. Background:MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.