Recombinant Human MSTN (E. coli)

Description

Growth differentiation Factor 8 GDF-8 , also known as myostatin, is a secreted protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass. GDF-8 belongs to the transforming growth factor beta TGF-beta superfamily, which includes the TGF-betas, bone morphogenetic proteins BMPs , growth differentiation factors GDFs , activins, inhibins, leftys, nodal, Mullerian inhibitory substance MIS and the glial cell line-derived neurotrophic factors GDNFs . All TGF-beta superfamily members are synthesized and secreted as a homodimeric prepropeptide that is cleaved by proprotein convertases such as furin to generate the dimeric N-terminal propeptide and the dimeric C-terminal mature active protein. The C-terminal mature protein contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Mouse GDF-8 cDNA encodes a 376 amino acid residue aa preproprotein with a putative 24 aa signal peptide, a 243 aa propeptide and a 109 aa mature protein. As is the case with most TGF-beta family proteins, GDF-8 is highly conserved across species. Mature human, mouse, rat, and cow GDF-8 share 100 % aa sequence identity. Among TGF-beta family members, GDF-8 is most closely related to GDF- 11/BMP-11. The two proteins share 65 % overall aa sequence, within their mature regions, the two proteins differ only by 11 aa.residues. Similarly to TGF-beta-1, 2, and 3, the GDF-8 homodimeric propeptide and mature protein remained non- covalently linked after proteolytic cleavage, and is released as a biologically inactive latent complex that does bind its receptor. In serum, GDF-8 has also been found to exist in a large latent complex that also included FLGR follistatin-related gene and GASP-1 growth and differentiation factor- associated serum protein-1 in addition to the propeptide. Recombinant GDF-8 propeptide is capable of associating with the active GDF-8 with high-affinity to reconstitute the latent complex and is potent GDF-8 antagonist.