Recombinant Human NTAQ1 (N-GST)

Description

EnkiLife provides the Recombinant Human NTAQ1 (N-GST) protein,aslo know as Protein N-terminal glutamine amidohydrolase,WDYHV1,Protein NH2-terminal glutamine deamidase,N-terminal Gln amidase,Nt(Q)-amidase,C8orf32,NTAQ1, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Human Protein N-terminal Glutamine Amidohydrolase is produced by our E.coli expression system and the target gene encoding Met1-Cys205 is expressed with a GST tag at the N-terminus.Accession:AAH08781.1. Expression Host:E.coli.Species:Human.Predicted Molecular Mass:49.8 KDa. Background:Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.