Recombinant Mouse CD45 (C-6His)

Description

EnkiLife provides the Recombinant Mouse CD45 (C-6His) protein,aslo know as B220,CD45 antigen,CD45,CD45R,LCA,L-CA,LY5,protein tyrosine phosphatase, receptor type, C,PTPRC,receptor-type tyrosine-protein phosphatase C, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Mouse Receptor-type Tyrosine-protein Phosphatase C is produced by our Mammalian expression system and the target gene encoding Gln26/xadLys566 is expressed with a 6His tag at the C-terminus.Accession:P06800. Expression Host:Human Cells.Species:Mouse .Predicted Molecular Mass:59.5 KDa. Background:Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin. CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148. CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders.