Recombinant Mouse IGFBP-5 (C-6His)

Description

EnkiLife provides the Recombinant Mouse IGFBP-5 (C-6His) protein,aslo know as BP-5,IGFBP-5,IGF-binding protein 5,Insulin-like growth factor-binding protein 5;, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Mouse Insulin-like Growth Factor-binding Protein 5 is produced by our Mammalian expression system and the target gene encoding Leu20-Glu271 is expressed with a 6His tag at the C-terminus.Accession:Q07079. Expression Host:Human Cells.Species:Mouse.Predicted Molecular Mass:29.3 KDa. Background:Mouse Insulin-like growth factor-binding protein 5(IGFBP-5) belongs to the superfamily of insulin-like growth factor (IGF) binding proteins. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. Mouse IGFBP-5 shows 97% aa sequence identity with those of human and rat IGFBP-5. It is expressed mostly in kidney, uterus and gastrocnemius muscle. It also expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracelluar matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.