Recombinant Mouse SHH (C-6His)

Description

EnkiLife provides the Recombinant Mouse SHH (C-6His) protein,aslo know as Sonic Hedgehog Protein,SHH,HHG-1,SHH, which features high purity, high activity, and high stability. It can be applied in scientific research, biopharmaceuticals, and other fields.Purity:Greater than 95% as determined by reducing SDS-PAGE;Endotoxin Level:<1 EU/µg as determined by LAL test.;Construction:Recombinant Mouse Sonic Hedgehog is produced by our Mammalian expression system and the target gene encoding Cys25/xadGly198 is expressed with a 6His tag at the C-terminus.Accession:Q62226. Expression Host:Human Cells.Species:Mouse.Predicted Molecular Mass:20.4 KDa. Background:Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N/xadterminal fragment (Shh/xadN), and a catalytic/xadprocessing,glycosylated, 34 kDa, 239 aa C/xadterminal fragment. The 20 kDa Shh/xadN fragment is the core of the active hedgehog molecule. Mouse Shh/xadN is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh/xadN, respectively.