Recombinant RNase Inhibitor ver.2.0

Description

Recombinant RNase Inhibitor ver.2.0 contains a mutation in a cysteine residue that is very sensitive to oxidation, making it highly stable in enzymatic reactions requiring low DTT concentrations. It inhibits RNase A activity through the formation of a 1:1 complex in a reversible reaction. It is not effective against RNase H activity. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Comparison of oxidation resistance among different commercially-available recombinant RNase inhibitors.16S/23S rRNA was mixed with DTT-free buffer, RNase A, increasing amounts of H2O2, and either Recombinant RNase Inhibitor ver.2.0 Cat. # 2135; purple line , Recombinant RNase Inhibitor Cat. # 2313; blue line , or a commercially-available, oxidation-resistant murine RNase inhibitor Company N; green line . After incubation at 37C for 10 min followed by incubation at 25C for 10 min, each reaction was centrifuged at 13,000gfor 15 min at 4C. Nucleic acids released into the supernatant were measured as an indicator of RNase A-mediated rRNA degradation using a NanoDrop spectrophotometer. At concentrations above 0.1 % H2O2, the use of Recombinant RNase Inhibitor ver.2.0 resulted in less rRNA degradation compared to other RNase inhibitor products.n=2, where each experiment was performed in triplicate.