Recombinant Staphylococcus aureus Protein A

Description

Ensure the validity of your result using our recombinant S. aureus Protein A ab52953 to probe and detect IgG in SDS-PAGE. Check out our protein gel staining guide for SDS-PAGE here Function The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for Protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3 . It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep. This product is manufactured by BioVision, an Abcam company and was previously called 6500 Protein A Liquid form . 6500-10 is the same size as the 10 mg size of ab52953.