Phospho-Ksr-1 (Ser392) Rabbit Polyclonal Antibody

Description

EnkiLife offers the Phospho-Ksr-1 (Ser392) Rabbit Polyclonal Antibody. This antibody reacts with Human,Mouse species and is suitable for WB,IHC,ICC/IF,ELISA application. Alternative Names of Immunogen: KSR1; KSR; Kinase suppressor of Ras 1. The product boasts remarkable high specificity, outstanding reproducibility, and exceptional affinity, making it a reliable and superior choice for your needs. Dilution Ratio: WB 1:500-1:2000,IHC 1:100-1:300,ICC/IF 1:50-1:200,ELISA 1:10000-1:20000 Background: caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. similarity:Contains 1 phorbol-ester/DAG-type zinc finger. similarity:Contains 1 protein kinase domain. subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.