Retro-XTM Concentrator

Description

The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10100 fold, depending upon the volumes and envelope used. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Retroviral vectors available from Takara Bio.Not all vectors are shown. Some retroviral vectors are not sold separately. Back Back Far simpler than ultracentrifugation.In a side-by-side comparison of Retro-X Concentrator and ultracentrifugation, the advantages of the Retro-X method are clearly evident. Retro-X Concentrator is more flexible and easier to use than ultracentrifugation. The Retro-X Concentrator reagent is itself a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalability has been successful for supernatant volumes up to 250 ml. Back Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes.A retrovirus expressing DsRed2 was packaged with different retroviral envelopes using the Retro-X Universal Packaging System Cat. # 631530 , and harvested at 72 hr posttransfection. Retro-X Concentrator was used to concentrate dualtropic 10A1 , amphotropic Ampho , ecotropic Eco , and VSV-G pantropic pseudotyped retrovirus samples from 10 ml down to 100 mul Panels A&B . Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of positive cells. Back