Retro-XTM Tet-Off Advanced Inducible Expression System

Description

The Retro-X Tet-Off Advanced Inducible Expression System is a tetracycline-inducible retroviral gene expression system, consisting of retroviral versions of the Tet-Off Advanced regulatory and response vectors. Retroviruses produced from these vectors can be used to generate stable mammalian cell lines that yield high-level, inducible expression of cloned genes under the control of doxycycline. The kit also includes a control vector, a CalPhos Mammalian Transfection Kit, and Tet System Approved FBS. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Retroviral delivery combined with tetracycline-inducible expression.HeLa cells stably transduced with Retro-X Tet-Off Advanced retrovirus were subsequently infected with a Retro-X-Tight-Pur retrovirus engineered for inducible luciferase expression. Infected cells were grown in the absence or presence + of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units. Back Each Retro-X Tet-Advanced system consists of two retroviral vectors.A regulator vector e.g., pRetroX-Tet-On Advanced expresses an optimized tetracycline-controlled transactivator. The response vector pRetroX-Tight-Pur contains an improved tetracycline response element TRE within thePTightpromoter that controls expression of your gene of interest. Back