RetroNectin Precoated Dish

Description

Pack of ten 35 mm dishes that are pre-coated with RetronNectin reagent. RetroNectin reagent is a 63 kD fragment of recombinant human fibronectin fragment also referred to as rFN-CH-296 that enhances the efficiency of lentiviral- and retroviral-mediated gene transduction. This is particularly important for hematopoietic cells and other hard-to-transfect cell types. Enhanced transduction is thought to result from co-localization of virus particles and target cells. This is accomplished by direct binding of viral particles to sequences in the heparin-binding domain and interaction of target-cell integrins with two other domains in rFN-CH-296. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Schematic protocol for infecting target cells using RetroNectin reagent.In the RBV RetroNectin-bound virus method, the retrovirus is first bound to the RetroNectin-coated plate, and cells are added after removing the retrovirus supernatant. In this protocol, removal of the supernatant reduces inhibitory molecules that otherwise can reduce the transduction efficiency. Back Comparison of T cell expansion efficiencies for various commonly used protocols.Cells co-stimulated using RetroNectin and alpha-CD3 demonstrated higher-fold expansion as compared to other protocols Panel A . In addition, the presence of RetroNectin promoted the production of more naive-like T cells CCR7+/CD45RA+ phenotype that have been shown to have better in vivo anti-tumor activity Panel B . NOTE:This expansion method requires a license for commercial applications. Back Comparison of retroviral transduction of a K562 cell line using transduction enhancers from different companies.A K562 cell line was transduced with a retroviral vector in the presence of various transduction enhancers. RetroNectin yielded a far higher transduction efficiency relative to each of the other transduction enhancers, including Polybrene, protamine sulfate, and human fibronectin from three other companies. Back Efficient transduction of human PBMCs using RetroNectin, as compared to Polybrene.Human PBMCs were transduced with varying dilutions of lentivirus encodingZsGreen1in the presence of RetroNectin or Polybrene, and the percentages of transduced CD8+ cells were measured by flow cytometry. Transduction efficiencies with Retronectin were significantly higher than those obtained using Polybrene, especially at low MOI 90X dilution . Back T-cell expansion using RetroNectin reagent.Peripheral blood T cells were stimulated and expanded in vitro using anti-CD3 clone OKT3 and IL-2 alone or in combination with RetroNectin reagent RN , anti-CD28, or anti-4-1BB. Stimulation with RetroNectin reagent yielded higher proportions of cytotoxic CD8+ T cells. Back