RiboGoneTM - Mammalian

Description

RiboGone - Mammalian allows for the specific removal of rRNA sequences 5S, 5.8S, 18S,and28S , as well as mitochondrial rRNA sequences 12S , from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts 10100 ng of total RNA , and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis.RNA-seq libraries were generated from Human Brain Total RNA Takara Bio or Breast Cancer FFPE RNA Cureline, extracted using a NucleoSpin totalRNA FFPE kit and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo dT -enriched RNA Takara Bio , while retaining more noncoding reads. Back Back Back Back