RNA PCR Kit (AMV) Version 3.0

Description

The RNA PCR Kit AMV Version 3.0 is designed for performing single tube reverse transcription of RNA to cDNA using AMV Avian Myeloblastosis Virus Reverse Transcriptase, with subsequent cDNA amplification using TakaraEx TaqHS. Taking advantage of AMV RTase XL, which possesses higher thermostability and a broader range of reaction temperatures 42-60C than MMLV RTase, this kit allows transcription at higher temperatures, which eliminates potential RNA secondary structure that can give rise to polymerase pauses. The supplied Oligo dT-Adaptor Primer is designed for highly efficient cDNA synthesis from the poly A +RNA 3-terminus, which allows amplification of unknown 3-termini using 3-RACE. The components for the 3-RACE method are supplied in the kit. Cat. # RR019B contains 2 of Cat. # RR019A. Please refer to Cat. # RR019A for complete product documentation and resources. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.