SABOURAUD DEXTROSE BROTH, MODIFIED

Description

Microorganisms have the ability to grow and reproduce in cosmetics. Through this, they can bring spoilage and chemical changes to the product which in turn can cause even injury to the end user. Most important methods for isolation of microorganisms from cosmetic products include direct colony counts and enrichment culturing. Water insoluble products need to be rendered miscible before the isolation procedures. Dissolved products are further diluted and plated on appropriate broth/ agar. The isolated microorganisms are identified by routine microbiological methods or by commercial identification kits. Sabouraud Dextrose Broth, Modified is prepared in accordance FDA BAM for the cultivation of yeasts and molds from cosmetics. Sabouraud dextrose media are peptone media supplemented with dextrose to support the growth of fungi. 10-1dilution of respected sample is prepared in Latheen Broth, Modified, as per the BAM protocol. For enrichments, dilute prepared sample decimally in Sabouraud Dextrose Broth, Modified and incubate at 30?2?C for 48 h. If growth occurs, streak on Sabouraud’s dextrose agar, Malt Agar, w/ 2% Agar or Potato Dextrose Agar w/2% Agar and proceed for identification and confirmation.