Shield1

Description

After reconstitution in your solvent of choice, the Shield1 compound stabilizes a protein of interest tagged with the destabilization domain DD , which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of the stabilized protein of interestin vivo. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Ligand-dependent, targeted and reversible protein stabilization.A small destabilization domain DD; blue is fused to a target protein of interest. The small membrane-permeable ligand Shield1 red binds to the DD and protects it from proteasomal degradation. Removal of Shield1 however, causes rapid degradation of the entire fusion protein. The default pathway for the ProteoTuner systems is degradation of the fusion protein unless Shield1 is present. Back Back DD fusions exhibit predictable, dose-dependent protein stabilization by Shield1; using the DD optimal for your protein is crucial in getting the best performance.While DD-N solid blue line is suitable for N-terminal fusions and even C-terminal fusions for many proteins, DD-C dashed blue line is optimal for C-terminal fusions and for those proteins that do not tolerate N-terminal fusions. HEK 293 cells expressing the DD fusions noted in the figure legend were treated with varying concentrations of Shield1. 12 hr later, the amount of AcGPF1 stabilized by different concentrations of Shield1 was detected by fluorescence intensity using flow cytometry. DD-C at the C-terminus of AcGFP showed a lower basal level of protein in the absence of Shield1 purple line; see inset figure . MFI = mean fluorescence intensity. Back