SMART MMLV Reverse Transcriptase

Description

SMART MMLV Reverse Transcriptase is an ultra-pure, recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT . This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases. As a result, SMART MMLV RT is able to synthesize a higher percentage of full-length cDNAs, making it the ideal enzyme for a wide range of applications. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back Back SMART MMLV RT synthesizes long, full-length cDNAs.SMART MMLV RT was used to reverse transcribe first-strand cDNA from Human Brain Total RNA using oligo dT 18 primers Panel A . The resulting single-stranded cDNA was then serially diluted fivefold corresponding to 25 ng1.6 pg of RNA and analyzed by qPCR using QTaq DNA Polymerase Mix and gene-specific primers that amplified a 226-bp region near the 5 end of NF1 Panel B . The successful amplification of this region indicates that SMART MMLV RT was able to reverse transcribe at least 11.7 kb of the NF1 gene. Back