SMART-Seq Pro Application Kit - 2 Chip

Description

SMART-Seq Pro Application kit contains the necessary chips, reagents, and consumables required to run two complete experiments of single-cell, full-length transcriptome analysis on theICELL8 cx Single-Cell System Cat. No. 640188 . The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera technology for Illumina library preparation with dual 72 x 72 indexes Nextera Tagmentation bead-linked transposome not included . The protocols generate indexed libraries that are ready for sequencing on Illumina platforms. Provided index plates allow for two chip libraries to be processed simultaneously in a single sequencer run. ICELL8/MSND 384-Well Source Plate and Seals Cat. Nos. 640192, 640018, or 640037 need to be purchased separately. A total of four plates and seals are required to process each individual chip. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Comparison of genes detected from PBMCs using SMART-Seq Pro or Smart-seq2 SS2 workflows. Back Comparison of isoform data generated using the SMART-Seq Pro kit or 10x Chromium. The SMART-Seq Pro method using the ICELL8 cx system can identify junction reads across exons 3, 4, 5, 6, and 7 and shows distinct profiles forPTPRC-201andPTPRC-209isoforms. Data generated from 10x Chromium end-counting methods could not identify junctions in these same exon regions, with peaks representing noise from misaligned reads. Back