SMART-Seq Total RNA Pico Input (ZapR Mammalian)

Description

SMART-Seq Total RNA Pico Input ZapR Mammalian generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg10 ng inputs of purified total RNA. The protocol for use with this kit was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNAtypically present in high abundance following cDNA synthesis from total RNA inputsmakes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Enough material is provided with this kit to perform up to 384 4 x 96 reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Efficient depletion of rRNA-derived cDNAs across a wide input range.SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit sold as part of SMART-Seq Total RNA Pico Input ZapR Mammalian and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106paired-end reads. The bar graph Panel A and table Panel B show the read distribution of 250 pg-input and 10 ng-input libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to 250 pg-input untreated libraries. Back Superior gene and transcript detection across a wide input range.SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit sold as part of SMART-Seq Total RNA Pico Input ZapR Mammalian and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106paired-end reads. Gene counts Panel A and gene and transcript counts Panel B are increased for ZapR-treated libraries. Back Preservation of gene abundance information across a wide input range. SMART-Seq Total RNA Pico Input ZapR Mammalian was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Data analysis was performed with CogentAP using 2 x 106paired-end reads. The scatterplot illustrates the high correlation between the 10 ng-input libraries and 250 pg-input libraries. Required Products The Unique Dual Index UDI Kit 196 contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bios RNA-seq and DNA-seq kits. Back The Unique Dual Index UDI Kit 97192 contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bios RNA-seq and DNA-seq kits. Back The Unique Dual Index UDI Kit 193288 contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bios RNA-seq and DNA-seq kits. Back The Unique Dual Index UDI Kit 289384 contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bios RNA-seq and DNA-seq kits. Back The Unique Dual Index UDI Kit 124 contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit 196 , Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bios RNA-seq and DNA-seq kits. Back