SMARTer Human BCR IgG IgM H/K/L Profiling Kit

Description

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit enables users to analyze B-cell receptor BCR repertoires from total RNA samples. This kit can generate libraries from 10 ng to 1 mug of total RNA from peripheral blood mononuclear cells PBMCs and 1 to 100 ng of total RNA from B cells. This kit may be used for other sample types, but the protocol may need to be modified. The kit can be used to generate data for both heavy and light chains kappa and lambda of human immunoglobulin IgG and IgM. The SMARTer Human BCR IgG IgM H/K/L Profiling Kit leverages SMART technology and employs a 5 RACE-like approach to capture complete V D J variable regions of BCR transcripts. It also incorporates unique molecular identifiers UMI to facilitate PCR error correction and clonotype quantification during data analysis. Included in the kit are primers that incorporate Illumina-specific adapter sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms.This kit supports up to 12 rxns. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back