SMARTer RACE 5"/3" Kit

Description

The SMARTer RACE 5/3 Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART SwitchingMechanismAt 5 End ofRNATemplate technology, and facilitates the performance of 5- and 3-RACE Rapid Amplification of cDNA Ends PCR with the kits Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5 ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5 sequences, thus increasing the likelihood you will amplify the entire sequence of your gene. RACE PCR products are amplified with the provided SeqAmp DNA Polymerase, and cloned into the linearized pRACE vector with In-Fusion HD Cloning. The SMARTer RACE 5/3 Kit has been improved to accommodate larger RNA input volumes and perform better on challenging targets than the original SMARTer RACE cDNA Amplification Kit. The In-Fusion HD Cloning Kit, NucleoSpin Gel and PCR Clean-Up Kit, and Stellar Competent Cells are included for your convenience in cloning RACE products. Gene-specific RACE primers are supplied by the user. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back