SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian Components

Description

The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian Components are used with our SMARTer RNA Unique Dual Indexing Kits Cat. #s 634451, 634452, and 634457 to generate strand-specific RNA-seq libraries for Illumina sequencing from 250 pg10 ng inputs of purified total RNA. This kit incorporates Takara Bios proprietary SMART Switching Mechanism at the 5 end of RNA Template technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. This method was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNAtypically present in high abundance following cDNA synthesis from total RNA inputsmakes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. The new library design featured in the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian improves sequencing performance compared to the original SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian, particularly for NextSeq and MiniSeq instruments carrying the two-channel SBS technology. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back