SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian

Description

The SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing from 250 pg10 ng inputs of purified total RNA or from 101,000 intact cells. This kit incorporates Takara Bios proprietary SMART Switching Mechanism at the 5 end of RNA Template technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. This method was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNAtypically present in high abundance following cDNA synthesis from total RNA inputsmakes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. The new library design featured in this updated kit adds an 8 nucleotide nt unique molecular identifier UMI through the reverse-transcription step to mitigate potential PCR bias as well as to provide customers with additional information for transcript quantification, specifically for true variants and rare mutations. This kit includes the SMARTer RNA Unique Dual Index Kit - 24U Takara Bio, Cat. No. 634451 . SMARTer RNA Unique Dual Index Kit 96U Set A and B Cat. #s 634452, and 634457 are available for purchase. Together they offer up to 192 unique dual indexes for multiplexing. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back