SMCR8 CRISPR Knockout HeLa Cell Line

Description

This HeLa cell line features a stable knockout of SMCR8 mediated though CRISPR/Cas9 genome editing technology. This knockout cell line revealed critical insights into how SMCR8 interacts with C9orf72, localizes to lysosomes, and regulates mTORC1 signaling. The knockout of SMCR8 resulted in phenotypes such as lysosomal swelling and altered mTORC1 activity, underscoring its importance in lysosomal function and cellular homeostasis. This cell line is useful for studies investigating the interplay between SMCR8 and C9orf72 in regulating autophagy and lysosomal dynamics, which are crucial for understanding neurodegenerative diseases like ALS and FTD. Complete list of cell lines from this panel: Cat. T3807 - C9orf72 CRISPR Knockout HeLa Cell Line Cat. T3808 - SMCR8 CRISPR Knockout HeLa Cell Line Cat. T3809 - C9orf72-SMCR8 CRISPR Double Knockout HeLa Cell Line