Stable Mgat2 Knockout K16 CHO Cell Line

Description

Using CRISPR technology Mgat2 was silenced from the K16 CHO cells to generate a glycosylation mutant cell line which expresses hybrid type N-glycans. Mgat2 synthesizes the Mannosyl Alpha-1,6- -Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase enzyme GlcNAcT-II which plays a key role in the development of mammals in which carbohydrate-dependent interactions facilitate cell signaling, adhesion and migration. Carbohydrate-carbohydrate interaction by the hybrid type N-glycans has been shown to induce faster cell-cell adhesion and cell migration. Further research and discovery on the functions of N-glycans can be assessed with the Stable Mgat2 Knockout K16 CHO Cell Line.