T-Vector pMDTM19 (Simple)

Description

T-Vector pMD19 Simple is a linearized vector with a single 3-terminal thymidine at both ends. The T-overhang ends at the cloning site improve the efficiency of ligation of PCR products which contain A-overhangs at 3 -ends. The multiple cloning sites in thelac Zgene sequence have been deleted from this vector, but its beta-galactosidase activity is not disrupted. Therefore, transformant colonies containing recombinant plasmids can be identified by blue/white screening. Due to lack of multiple cloning sites in the vector, the inserted PCR product cannot be recut using restriction enzymes. When the insert DNA fragment is recut from the vector, the PCR primers should be used to add a restriction site to the 5-end for restriction enzyme digestion and subcloning. T-Vector pMD19 Simple is supplied with a Control Insert 500 bp DNA for positive control reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back