T-Vector pMDTM20

Description

T-Vector pMD20 is a linearized vector with a single 3-terminal thymidine at both ends. The T-overhang ends at the cloning site improves the efficiency of ligation of PCR products which contain A-overhangs at 3-ends. T-Vector pMD20 is derived from a pUC19 backbone with modifications in the coding sequence to reduce the false positive rate and improve the positive colonies screened by blue/white color on ampicillin/IPTG/X-Gal LB plates. In addition, an SP6 promoter has been inserted upstream of the multiple cloning site. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase Cat. #2520 . T-Vector pMD20 is supplied with a Control Insert 500 bp DNA for positive control reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back